Coding

Part:BBa_K4825052:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)


JF-Cry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 268
    Illegal BglII site found at 411
    Illegal XhoI site found at 244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

Bacillus thuringiensis，Kluyveromyces marxianus

References

1. Zhao Y, Liang S, Huang K, Huang R. Construction of A Set of Secreting Expression Vectors for Saccharomyces cerevisiae. Acta Microbiologica Sinica, Vol.42, No.4. August 2002. 2. 孙明, 郭素霞, 王鹏霞, 彭东海, 阮丽芳, 喻子牛. Nematicidal bacillus thuringiensis crystal protein gene cry1518-35. WO2010078708A1 [P]. 2010-07-15.